Amazing that this can be achieved at such a low cost. The DVD optical pickup is truly a marvel of engineering, incorporating fairly complicated optics into such a small space. The focusing lens additionally has a electromagnetically controlled suspension system for adjusting the focus, so in theory you should be able to achieve z positioning of the microspheres as well. It would be great if the red dvd laser could be filtered out and the microscopic fluctuations of the trapped particle could be imaged with the camera to estimate the trap stiffness.
A similar optical pickup made of optical components from newport or thorlabs would run you a few thousand dollars, whereas these dvd optical pickups can be had for $10-20.
Here is another great paper (and accompanying youtube video) that shows optical trapping, atomic force microscopy, and even imaging with a dvd optical pickup.
https://pubs.acs.org/doi/10.1021/acssensors.8b00340
https://www.youtube.com/watch?v=5bqujaldaCQ